Figure 9
From: Cinnamic acid induces apoptotic cell death and cytoskeleton disruption in human melanoma cells
M30 and tubulin labeling in HT-144 cells. HT-144 cells were treated with 0.4 or 3.2 mM cinnamic acid for 24 or 48 hours. Fragmented cytokeratin 18 (green) were labeled with M30 antibody FITC and microtubules (blue) were labeled with anti-α and β tubulin and secondary antibody TRITC-conjugated. A,B) cells with intact microtubules and M30(+); C) cells with microtubule disruption and M30(+); D) cells with microtubule disruption and M30(–). Arrows = M30 staining. The results demonstrate that cell death and microtubule disorganization are independent events in our system. The images were obtained by Laser Scanning Confocal Microscopy.