Figure 1

MTA1 knockdown leads to the upregulation of miR-125b level in NSCLC cells. A. Quantification of MTA1 mRNA level by quantitative RT-PCR in 95D and SPC-A-1 cells untransfected, transfected with MTA1 shRNA or control shRNA. B. Western blot analysis of MTA1 protein level in 95D and SPC-A-1 cells untransfected, transfected with MTA1 shRNA or control shRNA. B-actin was loading control. C. Quantification of miR-125b level by quantitative RT-PCR in 95D and SPC-A-1 cells transfected with MTA1 shRNA or control shRNA. D. Quantification of miR-125b level by quantitative RT-PCR in 95D and SPC-A-1 cells transfected with MTA1 shRNA or control shRNA, together with miR-125b inhibitor or control. *P < 0.05, **P < 0.01 compared to the controls.