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Figure 6 | Journal of Experimental & Clinical Cancer Research

Figure 6

From: Let-7b expression determines response to chemotherapy through the regulation of Cyclin D1 in Glioblastoma

Figure 6

Let- 7b regulated cyclin D1 expression. (A) Prediction of Let-7b binding site in cyclin D1 3’-UTR by TargetScan. (B) U251 and U251R cells were transfected with Let-7b mimics or with scramble mimics (SCR). Then cisplatin expression was detected by western blot. (C) The cyclin D1-3′-UTR luciferase construct was co-transfected into U251 cells with indicated concentration of Let-7b mimics or with a scramble mimics (SCR) as negative control. Each sample’s luciferase activity was normalized to that of renilla, and results were expressed as mean±SD (n = 3) (*p < 0.05).

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