Figure 7

Knockdown of cyclin D1 expression increased cisplatin induced G1 arrest and apoptosis in U251R cells. (A) U251R cells were transfected with shRNA against cyclin D1 or scramble (SCR), and expression of cyclin D1 was validated by western blot. (B) Cells were treated with cisplatin 0.625 μg/mL for 48 hours, then cell cycle was detected by flow cytometry. (C) Cisplatin-induced apoptosis was assessed by Annexin V staining followed by flow cytometry.