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Figure 2 | Journal of Experimental & Clinical Cancer Research

Figure 2

From: Pharmacokinetics of combined gene therapy expressing constitutive human GM-CSF and hyperthermia-regulated human IL-12

Figure 2

hGM-CSF and hIL-12 expression in heat treated A549 and Hep3B cells. A549 and Hep3B cells in 24-well plates were infected with Adcmv-hGMCSF-hsp-hIL12 virus for 24 hrs and heated at 45°C for 45 min. Twenty-four hours late, medium was collected for hGM-CSF and hIL-12 measurement. A) hIL-12 expression under heating and no heating treatment. B) hGM-CSF expression under heating and no heating treatment. C) Relative hGM-CSF and hIL-12 expression in A549 cells. D) Relative hGM-CSF and hIL-12 expression in Hep3B cells. HT: heating treatment. N = 5 repeated experiments.

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