hGM-CSF and hIL-12 expression in Hep3B tumor tissues. Adcmv-GMCSF-hsp-hIL12 was intratumorly injected. Tumors were not heated, heated for 1 time, 2 time, and 3 times at 42°C for 40 min. Animals were sacrificed at different time point and tumor tissues were homogenized for hGM-CSF and hIL12 detection. A) hIL-12 expression in tumor tissues. B) hGM-CSF expression in tumor tissues. N = 5 mice per group.