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Table 5 Previous reports on EGFR mutation test from circulating free DNA

From: Detection of EGFR mutations in circulating free DNA by PNA-mediated PCR clamping

Year Authors Subjects DNA concentration Mutation test Detection rate
2006 Kimura H, et al. [16] Asian 70 ng/mL (range, 0–1720 ng/mL) SARMS 48.1% (13/27)
Female : 37%
Nonsmoker : N/A
ADC : 85%
ORR : 33%
2008 Maheswaran S, et al. [24] Western N/A SARMS 39% (7/18)
EGFR mutant patients
2009 He C, et al. [29] Asian N/A Mutant-enriched PCR 49.3% (66/134)
Female : 37%
Nonsmoker : 53%
ADC : 75%
2009 Bai H, et al. [28] Asian N/A dHPLC 34.3% (79/230)
Female : 46%
Nonsmoker : 55%
ADC : 74%
ORR : 36% (37/102)
2009 Mack PC, et al. [26] Western/Asian : 96/4% 2.3 ng/μL (range, 1–9 ng/μL ) SARMS 20% (10/49)
Female : 56%
Nonsmoker : 53%
ADC : 67%
2009 Kuang Y, et al. [25] Western 52.3 ng/μL (range, 10–163 ng/μL ) SARMS and WAVE/Surveyor 54% (29/54)
Female : 81.5%
Whole genome amplification
Nonsmoker : N/A
ADC : N/A
ORR : 56%
2010 Brevet M, et al. [31] Western N/A Mass spectrometry genotyping assay (Sequenom) and mutant-enriched PCR
Whole genome amplification
23.2% (10/31)
Female : 52%
Nonsmoker : 45%
ADC : 97%
2010 Jian G, et al. [27] Asian N/A Taqman PCR 23.2% (13/56)
Female : 46%
Nonsmoker : 58%
ADC : 78%
ORR : 30%
2011 Jiang B, et al. [30] Asian Minimum 4 ng/μL (range, 11–66 ng/μL ) Mutant-enriched PCR 31% (18/58)
Female : 31%
Nonsmoker : 38%
ADC : 72%
2011 Taniguchi K, et al. [32] Asian N/A BEAMing 72.7% (32/44)
EGFR mutant patients
This study Kim HR, et al. Asian 8.6 ng/μL PNA-based PCR clamping 16.7% (10/60)
Female : 65%
Nonsmoker : 2%
ADC : 88%
   ORR : 100%    
  1. Abbreviations: ADC adenocarcinoma, SARMS scorpion-amplified refractory mutation system, PCR polymerase chain reaction, dHPLC denaturing high-performance liquid chromatography, BEAMing beads, emulsion, amplification, and magnetics, PNA peptide nucleic acid.