Changes in the malignant properties of HCC cells under CM stimulation. (A) CM significantly promoted HCC cell proliferation (**P < 0.01 vs. EBM at 48 h) was measured by CCK8. (B) The expression of Ki67 in the nucleus of HCC cells. (C) Wound healing assays were performed with MHCC97H cells incubated by CM or EBM. The amount of migrating cells at the wound front was much higher than that in the control (**P < 0.01). (D) In the cell motility/invasion assay, the average numbers of penetrating HCC cells induced by CM were all obviously higher than those induced by EBM (***P < 0.001). (E) CM significantly increased the expression of HCC invasion/metastasis-associated genes in HCC cells compared with EBM (*P < 0.05). (F) High expression of MMP9 and MMP2 were confirmed in MHCC97H cells by immunofluorescent staining.