Caspases are not activated in-EA induced cell death. A498 cells cells were treated with 100 nM EA or 0.1% DMSO (control) for 43 h, or with 200 μM etoposide for 24 h. Cells were then harvested and stained with the FLICA reagent which only binds active caspases. Levels of active caspase were then determined by fluorescence (A). A498 cells were treated with 200 nM EA or with 0.1% DMSO (control) for 48 h and protein was extracted. Western blot analysis was performed using an anti-caspase-3 antibody. B-actin was probed as a control for protein loading (B).