Figure 4
From: Renal cancer-selective Englerin A induces multiple mechanisms of cell death and autophagy
Inhibition of autophagy does not affect EA-induced cell death. A498 cells were cultured in the presence and absence of 100 nM EA or, with EA and 1X NEAA in combination for 44 h. Autophagy was then determined by flow cytometry after staining with Cyto-ID® (A). A498 cells were treated with 150 nM EA, 0.1% DMSO, 1X NEAA, 200 μM VP16 or with 100 nM EA plus 1X NEAA for 46 h. Cell viability was then determined using the PrestoBlue® assay (B). A498 cells were treated as in (B) and then apoptosis was determined by measuring histone-associated DNA fragments by ELISA (C).