EA inhibits activation of AKT and ERK kinsases. A498 cells were cultured with 100 nM EA or with 0.1% DMSO (control) for the indicated times and protein was Isolated. Western blot analysis was performed as described under Methods using antibodies against AKT, ERK, and AMPK and their phosphorylated counterparts. B-actin was probed to control for protein loading. (+) control; Jurkat cell extract.