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Figure 3 | Journal of Experimental & Clinical Cancer Research

Figure 3

From: Activation of NF-κB by the RANKL/RANK system up-regulates snail and twist expressions and induces epithelial-to-mesenchymal transition in mammary tumor cell lines

Figure 3

RANKL induces the activation of NF-κB. (A) 4T1 and NMuMG cells were incubated with 100 ng/mL RANKL. At various time points, the cytoplasmic fractions and nuclear fractions were extracted and then subjected to SDS-PAGE/immunoblotting with anti-NF-κB p65, anti-phospho-ERK1/2, anti-phospho-Akt, anti-phospho-mTOR, anti-phospho-JNK, anti-phospho-STAT3, anti-ERK1/2, anti-Akt, anti-mTOR, anti-JNK, and anti-STAT3 antibodies. Anti-β-actin and anti-lamin antibodies were used as internal standards. (B) Quantification of the amount of NF-κB p65, phospho-ERK1/2, phospho-Akt, phospho-mTOR or phospho-STAT3, normalized to the amounts of the corresponding proteins, respectively. The results are representative of 5 independent experiments. *p < 0.01, compared to controls (ANOVA with Dunnett’s test).

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