Results of quantitative RT-PCR in 48 HCC cases. (a) Expression levels of DCDC2 mRNA examined by RT-PCR in 48 cases. The expression index [(DCDC2-tumor) × (GAPDH-normal)/(DCDC2-normal) × (GAPDH-tumor)] was calculated for all 48 cases. Expression index in methylated cases were significantly lower than unmethylated cases (P = 0.048). (b) Methylation in normal tissue did not show significant difference in expression index (P = 0.153).