Figure 6

Activation of DNA damage response. Human transformed BJ-EHLT fibroblasts were treated with 0.5 μM of 1, 2 and 3 for 24 hrs, then fixed and processed for IF analysis with anti-γH2AX antibody, and counterstained with DAPI to mark nuclei. a: Representative images of IF at 63× magnification. b: Histograms shows the percentage of γH2AX-positive cells scored by immunofluorescence analysis (*P < 0.05).