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Figure 7 | Journal of Experimental & Clinical Cancer Research

Figure 7

From: On and off-target effects of telomere uncapping G-quadruplex selective ligands based on pentacyclic acridinium salts

Figure 7

Induction of telomere damage and aberrations. Cells untreated or treated with 1, 2 and 3 for 24 hrs were fixed and processed for IF analysis against TRF1 and γH2AX. a: Representative images of IF were acquired with a Leica deconvolution microscope (magnification 100×). Enlarged views (2.5×) of treated merged images are reported. Histograms represent the Percentage of TIFs-positive cells. b: and average number of TIFs per nucleus. c: in the untreated (vehicle) and treated cells with the indicated compounds. Cells with four or more γH2AX/TRF1 foci were scored as TIF-positive. d: BJ-EHLT fibroblasts treated with 1, 2 or 3 for 72 hrs at 0.5 μM were blocked in metaphase with colcemide and processed for FISH with a telomere specific probe. The average number of specific telomeric aberrations in each sample is reported in the histograms. In the lower panels, representative images of telomere aberrations are shown at 100× magnification. e: BJ-EHLT treated with 1, 2 or 3 for 24 hrs at 0.5 μM were fixed and stained with DAPI. Anaphase bridges were scored by counting 400 nuclei per sample in triplicate. Histograms show the fold induction in treated versus untreated samples. Right panels show representative images of anaphase bridges in the treated samples at 63× magnification. Histograms show the mean of three independent experiments. Error bars indicate ± SD. (*P < 0.05).

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