Figure 3

EZH2 knockdown inhibits the cell growth of SUM149 and FC-IBC-02S cells and tumor spheroid formation of FC-IBC-02S cells. A. SUM149 and FC-IBC-02S cells were infected with 2 individual lentivirus encoded shEZH2 or control. Expression of EZH2, H3K27Me3, H3K9Me3, and histone H3 was examined in drug-selected cells by western blotting. B. Same as A, 2000 of control or shEZH2 expressing FC-IBC-02S single cells were cultured in suspension in ultra-low attachment plates. After culturing 6 days, the number of tumor spheroids was counted. Mean of 3 independent experiments with SEM. *, P < 0.001 compared to control. C and D. Same as A, but 2 × 10³ of drug-selected cells were seeded and assayed by MTS in SUM149 (C) and FC-IBC-02S (D) cells at indicated time points. Mean of 3 independent experiments with SEM. *, P < 0.01 compared to control.