Figure 5

EZH2 knockdown suppresses the invasion and tumor angiogenesis of human IBC cells. A. Control and shEZH2 expressing SUM149 cells at equal number were seeded onto the noncoated membrane (migration) and matrigel-coated membrane (invasion). After incubating 24 hrs, the cells migrated through both uncoated and matrigel-coated membrane were stained with 0.05% crystal violet in PBS. B. Quantitation of A, relative percentage of shEZH2 expressing cells migrated through uncoated membrane or invaded through matrigel-coated membrane compared to controls was indicated. Mean of 3 independent experiments with SEM. *, P < 0.05 compared to control. C. Tumor tissues from FC-IBC-02S xenografts were stained with anti-CD31 antibody to measure microvessel density (×100 magnification). The CD31-positive microvessels are marked (arrows). Quantitation of CD31 vessel staining is shown in the bottom graph. *, P < 0.01 compared to control.