Figure 3

Different blood supply patterns in human glioma tissues and C6 glioma cell xenografts. A. Different blood supply patterns including EVs, MVs and VM in human gliomas. a) EVs in high grade gliomas (Black arrows point) (H&E × 200). b) Tumor cells (Large black arrow points) and endothelial cells (Small black arrow points) formed the structure of MV with red blood cells in it (H&E, ×400). c) VM in human high grade gliomas. Tumor cells formed the wall of VM (Black arrow points) with red blood cells in it (H&E, ×200). B. Double staining with CD31 IHC staining and PAS histochemical staining confirmed the wall structures of EVs, MVs and VM in human high grade gliomas. a) EVs were positive both for CD31 and PAS staining (Black arrows point) (×200). b) Tumor cells (CD31 negative staining, large black arrow points) and endothelial cells (CD31 positive staining, small black arrow points) formed the MV (×200). c) The wall of VM (black arrow points) was negative for CD31 staining and positive for PAS staining (×200). C. MVs, VM and PGCCs in human glioma cancer cell line C6 xenograft of chicken embryonating eggs. a) The gross imagine of the embryonating egg xenograft model (Black arrow point the tumor mass). b and c) VM in C6 xenografts with nucleated red blood cells in it (Black arrows point) (HE,×200). d) Tumor cells (Black arrow points) and endothelial cells (Blue arrow points) formed the structure of MVs with nucleated red blood cells in it (H&E, ×200). e and f) Presence of PGCCs in the embryonating eggs xenografts (Black arrows point) (H&E, ×200).