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Figure 3 | Journal of Experimental & Clinical Cancer Research

Figure 3

From: Kaposi sarcoma associated herpesvirus (KSHV) induces AKT hyperphosphorylation, bortezomib-resistance and GLUT-1 plasma membrane exposure in THP-1 monocytic cell line

Figure 3

GLUT1 membrane exposure, induced by KSHV infection of THP-1 cells, increases after Bortezomib treatment. A) GLUT1 Immunofluorescence in mock and KSHV-infected THP-1 cells in the presence of Bortezomib (Bz), LY294002 (Ly) or the combination of them (Ly + Bz). GLUT1 staining (red) is mainly accumulated at the membranes on ~ 15% of KSHV-infected cells mock treated and in ~ 40% of the KSHV-infected cells upon bortezomib treatment. The counterstaining of THP-1 DNA with DAPI (blue) is shown. B) Western blot analysis showing the expression of GLUT1 in membrane fraction of mock and KSHV-infected THP-1 cells untreated or treated with bortezomib (Bz), LY294002 (Ly) or both (Ly + Bz). Ponceau staining of the membrane is reported as loading control.

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