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Figure 5 | Journal of Experimental & Clinical Cancer Research

Figure 5

From: IL-27 inhibits epithelial-mesenchymal transition and angiogenic factor production in a STAT1-dominant pathway in human non-small cell lung cancer

Figure 5

Inhibition of in vitro cell migration dependent upon STAT1 activation. (A) A549 cells were treated with IL-27 (50 ng/mL) at 60 ~ 70% confluency for 24 hours and a scratch was created in the cell monolayer. The same fields were observed for cell migration using phase contrast microscopy after 24 hours of IL-27 treatment. (B) The scratch technique was utilized to measure cell migration for A549 cells that were transfected with STAT1 siRNA (40 nM) for 24 hours prior to with or without IL-27 (50 ng/mL) exposure. (C) The motility assay was employed to measure cell migration after Stattic (7.5 nM) pre-treatment for 1 hour prior to IL-27 exposure (50 ng/mL), and changes in cell migration were observed for 60 hours. Scale bar, 200 μm. (D and E) Cell migration evaluated using transwell chambers. A549 sells transfected with STAT siRNAs for 24 hrs, control siRNA-transfected or untreated cells (D) followed by 1 hour of Stattic treatment (E) were plated 24 h after treatment with IL-27 on 96-well transwell plates. After 48 hours, cells that migrated through the pores to the under surface of the membrane and bottom wells were labeled with Calcein-AM. Migration rate was calculated using fluorescence as described in Materials and Methods.

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