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Figure 5 | Journal of Experimental & Clinical Cancer Research

Figure 5

From: Suppression of autophagy enhances preferential toxicity of paclitaxel to folliculin-deficient renal cancer cells

Figure 5

Beclin 1 knockdown inhibited autophagy and sensitized FLCN-deficient cells to paclitaxel. A. Cells were transfected with Beclin 1 siRNA or a random siRNA control for 24 hours and subsequently treated with 100 nM paclitaxel for 24 hours with or without bafilomycin A1 treatment, LC3 protein levels were detected using Western blot. Less LC3 proteins were detected in Beclin 1 siRNA treated cells. B. FLCN-deficient cells transfected with Beclin 1 siRNA or a random siRNA control were treated with different concentrations of paclitaxel. MTT assay showed that cell viability was obviously decreased after Beclin 1 siRNA treatment (*: p < 0.05. UOK257 + Paclitaxel + random siRNA vs UOK257 + Paclitaxel + beclin 1 siRNA; ACHN 5968 + Paclitaxel + random siRNA vs ACHN 5968 + Paclitaxel + beclin 1 siRNA; n = 15). C. Number of apoptotic cells increased after treatment with Beclin 1 siRNA and 100 nM paclitaxel (*: p < 0.05. UOK257: Paclitaxel + random siRNA vs Paclitaxel + beclin 1 siRNA; ACHN 5968: Paclitaxel + random siRNA vs Paclitaxel + beclin 1 siRNA; n = 15).

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