Figure 3

Regulation of TWIST 3′-untranslated region (UTR) by miR-33a. (A) MG-63 cells were co-transfected with a TWIST-3′UTR-luciferase reporter and miR-33a or control miR-Vec (hTR). The relative luciferase activity in cells co-transfected with hTR was designated as 1. ( B) Schematic presentation of generation of a TWIST-mut33-luciferase reporter (mut33) by site-directed mutagenesis of the predicted binding sequence of miR-33a in TWIST 3′-UTR. ( C) MG-63 cells were co-transfected with the TWIST-3′UTR-luciferase reporter or TWIST-mut33-luciferase reporter and miR-33a or control miR-Vec (hTR). The relative luciferase activity in cells co-transfected with the TWIST-3′UTR-luciferase reporter (wild type) and hTR was designated as 1. *p < 0.05 vs hTR.