Figure 4
miR-33a and TWIST levels in osteosarcoma (OS) tissues from chemoresistant and control OS patients in the validation cohort. Real-time RT-PCR and Western blot analysis were performed to determine (A) miR-33a and (B) TWIST protein levels in OS tissues from the chemoresistant OS and control groups in the validation cohort (n = 35 each group), respectively. Data are shown in scatter plots. The mean miR-33a and TWIST protein levels are marked by a horizontal bar in each group.