Figure 5

miR-33a and TWIST levels in osteosarcoma (OS) cells. Real-time RT-PCR and Western blot analysis were performed to determine (A) miR-33a and (B) TWIST mRNA and (C) TWIST protein levels in Saos-2 and MG-63 human OS cells. The miR-33a expression level and the relative TWIST mRNA level and protein blot density in MG-63 cells were designated as 1, respectively. *p < 0.05 vs MG-63.