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Figure 6 | Journal of Experimental & Clinical Cancer Research

Figure 6

From: miR-33a is up-regulated in chemoresistant osteosarcoma and promotes osteosarcoma cell resistance to cisplatin by down-regulating TWIST

Figure 6

TWIST expression in osteosarcoma cells with overexpression or knockdown/inhibition of TWIST and/or miR-33a. (A) In Saos-2 cells, TWIST expression was determined in normal control cells (NC, lane 1), cells stably transfected with empty pcDNA3 vector (VC, lane 2), cells overexpressing TWIST (lane 3), cells overexpressing miR-33a (lane 4), cells transfected with antagomir-33a (lane 5), cells overexpressing TWIST and miR-33a (lane 6), and cells overexpressing TWIST plus transfection of antagomir-33a (lane 7). (B) In MG-63 cells, TWIST expression was determined in normal control cells (NC, lane 1), cells stably transduced with scramble control shRNA (SC, lane 2), cells stably expressing TWIST-shRNA (T-shRNA, lane 3), cells overexpressing miR-33a (lane 4), cells transfected with antagomir-33a (lane 5), cells stably expressing T-shRNA and overexpressing miR-33a (lane 6), and cells stably expressing T-shRNA plus transfection of antagomir-33a (lane 7). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) blotting was used as a loading control. Density of the TWIST blot was normalized against that of GAPDH to obtain a relative blot density, which was expressed as fold changes to the relative TWIST blot density of NC cells (designated as 1). In (A) Saos-2 cells, ap < 0.05 vs NC and VC; bp < 0.05 vs TWIST; cp < 0.05 vs miR-33a; dp < 0.05 vs antagomir-33a. In (B) MG-63 cells, ap < 0.05 vs NC and SC; bp < 0.05 vs T-shRNA; cp < 0.05 vs miR-33a; dp < 0.05 vs antagomir-33a; ep < 0.05 vs T-shRNA + miR-33a.

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