Figure 5From: Geldanamycin-mediated inhibition of heat shock protein 90 partially activates dendritic cells, but interferes with their full maturation, accompanied by impaired upregulation of RelBGA affects TF activities, and reduces RelB expression in MO-DCs. (a) HEK293T cells were transfected with TF responsive luciferase reporter vectors. After 5 h, cells were split, and aliquots were differentially treated in triplicates with GA, and/or the MO-DC maturation cocktail as indicated. One day later, luciferase activities were detected. Data show the means ± SEM of three experiments, normalized to the relative luciferase activity of untreated HEK293T cells, arbitrarily set to 1. Statistical significance: *versus unstimulated untreated, and #GA-treated at stimulated versus unstimulated state, and $GA-treated versus untreated at stimulated state (*,$P < 0.05, **P < 0.01, ***,###P < 0.001). (b) Groups of MO-DCs were generated as described (see legend of Figure 2). Derived protein (each 30 μg) was separated on SDS-PAGE, and western blots were performed. β-actin served as loading control. The graph is representative of two independent experiments.Back to article page