Figure 5

Effects of CF on the survival pathway in HCT-116 and MSTO cells. Cells were cultured in the absence or presence of CF (1:200) for the indicated times and whole cell lysates were analyzed by western blot. Data representing three independent experiments with similar results, indicate a downregulation of Bcl-2 and p-AKT, whereas total AKT does not change in HCT-116 and MSTO treated with CF for 24 and 48 h vs untreated cells. γ tubulin was examined as a loading control.