Figure 3

CFLAR is down-regulated in parthenolide -induced apoptosis in a dose-dependent (A) and a time-dependent (B) manner, and overexpression of CFLAR _L can protect cells from parthenolide-induced apoptosis (C,D and E). The indicated cells were treated with indicated concentrations of PTL for 24 hrs (A) or treated with 20 μmol/L PTL for various lengths of time and harvested for Western blot analysis (B). Indicated cells were seeded in 6-well plates and on the second day treated with 20 μmol/L PTL for another 24 hours and harvested for Western blot analysis (C) or for detection of apoptotic cells using Annexin V/PI staining (D, E). Points:mean of three replicate determinations; bars: S.D. P value < 0.05.