Figure 7
From: Parthenolide induces apoptosis via TNFRSF10B and PMAIP1 pathways in human lung cancer cells
Parthenolide selectively inhibits cell growth (A) and induces stronger apoptosis (B) in A549/shCDH1 cells and apoptosis, and ER stress related proteins are up-regulated more clearly by parthenolide in A549/shCDH1 cells than that in control cells (C). Knockdown of DDIT3 decreases parthenolide–induced PMAIP1 and apoptosis (D). The indicated cell lines were seeded in 96-well plates and treated with the given concentration of PTL for 24 hrs (A). Live cell number was estimated using SRB assay for calculation of cell survival. Points: mean of four replicate determinations; bars: S.D. A549/shCtrl and A549 shCDH1 cells were treated with indicated concentrations of PTL for 24 hrs. Both attached and suspended cells were harvested for Western blot analysis; CF: cleaved form (B,C). A549/shCtrl and A549 shCDH1 cells were seeded in 6-well plates and on the second day transfected with control or DDIT3 siRNA. Cells were treated with 20 μmol/L PTL for 24 hours after 48 hrs of transfection and harvested for Western blot analysis (D).