Table 2 Summary of univariate and multivariate analyses of 5-hmC and IDH2 protein expression associated with survival and recurrence in the training cohort (N = 318)

Sex (female vs. male)

0.959

NA

0.083

NA

Age, years (≤50 vs. >50)

0.772

NA

0.597

NA

HBsAg (negative vs. positive)

0.983

NA

0.491

NA

AFP, ng/ml (≤20 vs. >20)

0.041

1.893

1.257–2.852

0.002

0.230

NA

γ-GT, U/L (≤54 vs. >54)

0.006

1.619

1.118–2.343

0.011

0.003

1.547

1.138–2.102

0.005

Liver cirrhosis (no vs. yes)

0.077

NA

0.009

1.824

1.135–2.930

0.013

Tumor number (single vs. multiple)

0.003

NS

0.002

1.651

1.135–2.402

0.009

Tumor size, cm (≤5 vs. >5)

0.009

NS

0.041

NS

Tumor encapsulation (complete vs. none)

0.261

NA

0.166

NA

Microvascular invasion (no vs. yes)

0.003

NS

0.001

1.775

1.287–2.448

<0.001

Tumor differentiation (I-II vs. III-IV)

0.138

NA

0.053

NA

TNM stage (I vs. II III)

<0.001

2.048

1.412–2.971

<0.001

<0.001

1.649

1.134–2.397

0.009

5-hmC (low vs. high)

<0.001

0.316

0.211–0.472

<0.001

0.001

0.462

0.335–0.636

<0.001

IDH2 (low vs. high)

<0.001

0.405

0.275–0.594

<0.001

0.006

0.591

0.432–0.810

0.001

Combination of 5-hmC and IDH2

<0.001

<0.001

<0.001

<0.001

I versus II

0.002

3.987

1.890–8.413

<0.001

0.001

2.651

1.576–4.461

<0.001

I versus III

0.002

3.359

1.607–7.025

0.001

0.003

2.098

1.247–3.530

0.005

I versus IV

<0.001

8.908

4.215–18.825

<0.001

<0.001

3.891

2.270–6.671

<0.001