Aberrant activation of the Shh pathway in lung SCC. (A) Representative protein expression of Shh, Smo, Ptch1 and Gli1 by IHC staining, scored as 0 (negative), 1 (mild positive), 2 (positive), and 3 (strong positive). (B) Expression distributions of Shh, Smo, Ptch1 and Gli1 in 177 patient tissue specimens in the Tianjin cohort. (C) Association between the expressions of Hh pathway components. Kendall’s tau-b statsitcs was used to determine the correlation between proteins. The correlation coefficient r and p values were presented in (C). Kappa test was also performed with IHC scores of 1–3 grouped as “+”, 0 as “-”. Kappa test’s p values were labeled with*.