Gli1 expression reversely correlates with E-Cadherin expression in lung SCC. (A) Expressions of Gli1 and E-Cadherin (E-Cad) in three representative tissue specimens in the UCSF cohort with Gli1 expression at a low level (upper panels) and high levels (middle and lower panels). (B) Expressions of Gli1, E-Cad and β-Catenin (β-Cat) in three representative tissue specimens in the Tianjin cohort with Gli1 expression at a low level (upper panels), a mixed expression pattern (middle panels) and a high level (lower panels). (C) Correlations between Gli1, EMT markers, and recurrence/metastasis. Statistical analysis was performed between Gli1 and E-Cad, Gli1 and β-Cat, Gli1 and recurrence/ metastasis. (D) Gli1 and E-Cad expression in four lung SCC cell lines by Western blots.