Figure 2

Percentage of suppression by each Treg subset on the proliferation of responder T cells. (A-D) CFSE dilution by 1 × 10⁴ labeled CD4⁺CD25^-CD45RA⁺ T cells (responder T cells) assessed after 86 hr of TCR-stimulated co-culture with indicated Treg subset at a 1 to 1 ratio. Flow plots for one representative HNSCC patient. Percentage of suppression is indicated. In each histogram, the lines indicate the parent population (parent line) and the generation population (generation line) 1, 2, 3… from right to left. (E) The histogram represents the mean percentages of suppression ± SD (n = 12). HNSCC: head and neck squamous cell carcinoma. Statistical comparisons were performed using the Student’s t-test.