Figure 3

Berberine increased the phosphorylation of p38 MAPK and ERK1/2 in a time-dependent manner. A-B, A549 cells were treated with BBR (25 μM) in the indicated times, and cell lysate was harvested and the expression of the phosphorylated or total protein of ERK1/2, p38 MAPK were measured by Western blot analysis using corresponding antibodies. GAPDH was used as loading control. The bar graphs represented the densitometry results of p-ERK (A) or p38 MAPK (B)/GAPDH as mean ± SD of at least three separate experiments. *indicates significant difference from untreated control cells (P < 0.05).