AQP3 facilitates GC cell migration and invasion. GC cell migration and invasion were detected using transwell migration and invasion assays. The number of cancer cells migrating through the Matrigel decreased significantly after treatment with RNAi AQP3 compared with the UNTR group, while treatment with EGF had the opposite effect (A and B). AQP3-silenced GC cells invaded significantly slower when compared with the UNTR group and over-expression of AQP3 accelerated cell invasion (C and D). Data are expressed as the mean ± SE from three independent experiments. #P < 0.05 compared with the untreated group (UNTR); *P < 0.05 compared with the RNAi AQP3 group. Original magnification × 100.