Validation of methylation status of 21 candidate genes and 18 CIMP markers. The methylation status of 21 candidate genes and 18 CIMP markers was validated using QMSP in 10 CRC tissues compared with adjacent normal tissues (A), normal colon cells, and three CRC cell lines (B). Methylated intensity ratio of QMSP data was determined by the PMR values. The significance of different methylation statuses in CRC tissues and adjacent normal tissues was defined with the analysis of variance test using Sigma Stat. Values of P < 0.05 were considered to indicate statistical significance. * indicates the methylation status of genes in CRC compared with adjacent normal tissues was not statistically significant.