Figure 3
Demethylating effect of 5-aza-dC and vincristine on methylated genes in three CRC cell lines. Changes in the methylation status of hypermethylated genes in normal colon cells (A) and CRC cells (B) were identified by QMSP after treatment with 30 μM 5-aza-dC or 100 nM vincristine for 48 h at 37°C. Methylated intensity ratio was determined by the percentage of methylated reference (PMR).