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Figure 4 | Journal of Experimental & Clinical Cancer Research

Figure 4

From: Identification of novel hypermethylated genes and demethylating effect of vincristine in colorectal cancer

Figure 4

Association between DNA methylation and mRNA expression of eight genes in CCD18Co and DLD-1. The DNA methylation status (A) and mRNA expression (B) of eight genes, including six newly identified hypermethylated genes, was measured using QMSP and RT-PCR in CCD18Co and DLD-1 cells following 30 μM 5-aza-dC or 100 nM vincristine treatment for 48 h at 37°C. ACTB was amplified simultaneously with the other PCR products and was used as a control for DNA methylation and mRNA integrity. Marker: molecular weight DNA marker.

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