Figure 7

HRM analysis of IDH1 mutations. 1) Melting curve profiles of IDH1 mutated and wt alleles. Vertical axis corresponds to changes in the fluorescence signal over time (dF/dT). Mutated alleles were shifted to lower temperatures, but differentiation between different mutations was not possible. 2) Difference plot for HRM analysis of IDH1 mutations normalised to wt allele, discrimination of different mutations was difficult because of similar graphs. 3) Difference plot for HRM analysis of IDH1 mutations normalised to the R132S C>A allele, determination of different mutations was easier because of clearly separated graphs.