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Figure 9 | Journal of Experimental & Clinical Cancer Research

Figure 9

From: Comparative examination of various PCR-based methods for DNMT3A and IDH1/2 mutations identification in acute myeloid leukemia

Figure 9

Possible diagnostic workflow to identify DNMT3A, IDH2 and IDH1 mutations in routine laboratory analysis. HRM analysis can be performed in the first diagnosis for all mutations because of high mutational ratios prior to therapy. Unclear results can be verified by endonuclease restriction or ARMS-PCR. Unclear IDH1 results can be checked by sequencing because of the heterogeneity of possible mutations. Effective combination of all the available methods enables more reliable results and a cost-effective and time-saving routine laboratory analysis.

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