Figure 5
Analysis of AMH, AMHRII expression and CytP450 activity. (A) Real-time PCR to assess the percentage of expression levels of AMH (1), AMH (2), AMH type II Receptor (1) and (2) (AMH RII) genes in endometrial epithelial and stromal cell line respectively. (B) Expression levels of the Cytochrome P4501 and 2 isoforms and Reverse Transcriptase–Polymerase Chain Reaction (RT-PCR) for the CytP (450) 1 and 2 in epithelial and stromal cell line respectively; GAPDH represents loading control. (C) CYP Activity assay in endometrial stromal cells treated for 24 hrs at 1000 ng/mL of MIS full-length. (D) CYP Activity assay in endometrial stromal cells treated for 24 hrs at 1000 ng/mL of Plasmin-cleaved MIS.