Figure 7

Effects of agonist (UII), antagonist (Urantide, UPG83 and UPG85) and superagonist (UPG84) on bladder cancer cell growth. T24 (A), RT112 (B) MCR (C) and HT1376 (D) cells were treated with UII, urantide, UPG83, UPG85 at concentrations ranging from 10 to 1,000 nM, and with UPG84 at concentrations ranging from 10 to 2,000 nM (E), for 72 h at 37°C as described in “Methods” section.% of cell growth was calculated respect untreated cells at 72 h from the beginning of the treatment. Same doses were used for the treatment at 48 h but in these cell lines, the 50% inhibition of cell growth was achieved only at high concentration (T24) or was not reached (RT112). Asterisks indicate the statistical significance of the data (P <0.005). (F) HT-1376 (grey) and T24 cells (black) were cultured in the presence or absence of either 10 nM urantide or UPG84 and the expression of UTR was evaluated at FACS at 48 and 72 h as reported in “Methods”. The intensity of UTR expression was represented as% Mean fluorescence intensity (MFI) calculated comparing the MFI of cells incubated with the compounds with those of not treated cells. Each value is the mean of at least three different determinations performed in three different experiments. Bars, SEs. The experiments were performed at least three different times and the results were always similar.