Figure 2

Butein induction of apoptosis. (A) Breast cancer cells were treated with butein at 0-100 μg/ml (left triangles) for 24 hours and then cleavages of PARP and Caspases were examined with appropriate antibodies in western blots. Actin was detected as a loading control. (B) Breast cancer cells were treated with butein at 10 μg/ml for 24 hours and then stained with Annexin V and PI. Cells were then counted using flow cytometry. Experiments were performed in triplicate and data present the mean ± SD.