The effects of extracellular ATP on the expression of p65 and OPN in NPC cells. A: 5-8 F or CNE-2 cells were incubated with ATP (100 μM), and western blotting and immunofluorescence were used to detect the expression of p65. A notable decrease in p65 in the nucleus of 5-8 F and CNE-2 cells treated with ATP was found. The intensity of the bands was quantified. B: 5-8 F or CNE-2 cells were incubated with ATP (100 μM) for the indicated time, and OPN expression was detected by western blotting, with quantification of the band intensity. Similar to the situation for p65 in NPC cells, ATP significantly downregulated OPN expression in 5-8 F and CNE-2 cells. Statistical significance between the control and treated conditions: *P < 0.05.