Effects of ATP and P2Y2 on the regulation of OPN by p65. A: Overexpression of P2Y2 downregulated the levels of p65 and OPN, and p65 knockdown with shRNA decreased the level of OPN in 5-8 F and CNE-2 cells. B: ATP and P2Y2 reduced the transcriptional activity of OPN promoter. C: ATP lowered the binding of p65 to the promoter region of OPN in 5-8 F and CNE-2 cells. The data represent the means ± SD of 3 independent experiments. Statistical significance between the control and treated conditions: *P < 0.05 (100 μM).