P-AKT was involved in the effects of ATP and P2Y2 in NPC cells. A: The Akt pathway was involved in the effect of ATP on NPC cells. ATP decreased the level of p-Akt in CNE-2 cells, and OPN downregulation decreased the level of p-Akt and enhanced the extent of the downregulation of p-Akt by ATP. The overexpression of OPN increased the level of p-Akt and mitigated the effect of ATP on the p-Akt level. B: The overexpression of P2Y2 could decrease the p-AKT level in CNE-2 cells. The level of p-Akt was detected by western blotting, with quantification of the band intensity. Statistical significance between the control and treated conditions: *P < 0.05.