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Figure 3 | Journal of Experimental & Clinical Cancer Research

Figure 3

From: A novel miR-219-SMC4-JAK2/Stat3 regulatory pathway in human hepatocellular carcinoma

Figure 3

SMC4 is a downstream target of miR-219. (A) The predicted binding sequence of SMC4 and its binding site in the 3’-untranslated region (UTR) of SMC4 (sense) is presented for alignment. (B) Real-time quantitative PCR results showing miR-219 levels in HCC tissues and cell lines (T, HCC tissues; P, paracancerous tissues; N, non-tumor tissues). (C) Real-time quantitative PCR result showing that SMC4 upregulation by miR-219-inhibit, and also downregulation by overexpression of miR-219 in 97-H and HepG2 cell lines. (D) Western Blotting result showing that SMC4 upregulation by miR-219-inhibit, and also downregulation by overexpression of miR-219 in 97-H and HepG2 cell lines (1,97-H/HepG2; 2, 97-H/HepG2-vector; 3, 97-H/HepG2-minics; 4, 97-H/HepG2-inhibitor). (E) Luciferase reporter assays were used to confirm the interaction of miR-219 with SMC4. 3’-UTR of SMC4 containing the target binding site (sense) was cloned downstream of a firefly luciferase gene. The plasmids were transfected into empty vector and miR-219 stably expressing cells (97-H, HepG2).

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