Figure 2
From: Characterization of the biological activity of a potent small molecule Hec1 inhibitor TAI-1
TAI-1 Disrupts Hec1-Nek2 interactions, induces chromosomal misalignment and induces apoptosis of cancer cells. (A) K562 cells were treated with 500 nM TAI-1, lysates immunoprecipitated with anti-Nek2 antibody were probed for Hec1 by western blotting to determine interaction. (B) K562 cells were treated with TAI-1 at 1 μM for the indicated time points and collected for immunoblotting of Hec1 and Nek2. (C) MDA-MB-468 cells treated with 1 μM TAI-1 were immunofluorescent stained for DNA and mitotic spindle. (D) Metaphase cells were counted for percentage of cells with misaligned chromosomes. (E) Lysates of HeLa treated with TAI-1 for 8 or 24 hours were western blotted for apoptotic markers caspase3 and PARP and anti-apoptotic markers MCL-1, XIAP, and BCL-2. Actin was used as loading control.