Figure 5

Inhibition of JNK expression downregulates Beclin 1 and represses autophagy. (A) BxPC-3 and PANC-1 cells were treated with different concentration of DHA for 24 h in the presence or absence of 10 μmol/L SP600125 pretreatment for 1 h. The expression levels of the LC3-I and LC3-II proteins were subsequently analyzed by immunoblotting. (B) BxPC-3 cells transfected with the GFP-LC3 plasmid, followed by 50 μmol/L DHA for 24 h with or without SP600125 (10 μmol/L). The number of GFP-LC3 dots was subsequently scored in 100 transfected cells. (C) BxPC-3 cells were treated with 50 μmol/L DHA for 24 h in the absence or presence of JNK1/2 siRNA. The expression levels of phospho-JNK and Beclin 1 protein were subsequently analyzed by immunoblotting. (D) BxPC-3 cells transfected with a non-targeting RNA or a JNK1/2-targeted siRNA were treated with 50 μmol/L DHA for 24 h. At the end of the treatment, cell viability was measured using a CCK-8 assay. *P < 0.05.