HDAC8 expression in urothelial cancer cell lines. (A) Relative mRNA expression of HDAC8 in eight urothelial cancer cell lines (UCCs) compared to two normal uroepithelial cultures (NUC; mean value set as 1) measured by quantitative RT-PCR. The HDAC8 expression values were adjusted to TBP as a reference gene and are displayed on the y-axis. The dotted line shows the average expression level of the NUC samples. (B) Protein expression of HDAC8 in urothelial cancer cell lines (UCCs) and a normal uroepithelial control (NUC) analyzed by western blotting. As a loading control α-tubulin was stained on each blot.